Prof. Liang Tang

SRI International, Menlo Park, California, USA

Title:

Enhancement of Sensitivity and Quantification Quality in the LC MS/MS  Measurement of Large Biomolecules with Sum of MRM (SMRM)

Abstract: 

Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) has become a mainstay  analytical technique in pharmaceutical industry and biomedical research. There was a challenge for  large molecules such as proteins and peptides in LC-MS/MS analysis, because they can exist in multiple  charged forms, and this will reduce total analyte signal by distributing it into multiple ion peaks with  different number of charges in a mass spectrum. This presentation will discuss innovative technique to  analyze large biomolecules by LC-MS/MS. The novel SMRM technique and application of Charge Conveyance Effect of large biomolecules can  enhance detection sensitivity to measure potent macromolecule drugs and biomarkers at trace-levels,  expedite method development in the bioanalysis of large biomolecules, facilitate the application of LCMS/MS in biotransformation research of large biomolecule drugs, enable the separation and  quantification of large-biomolecule isomers individually, and enable LC-MS/MS to expand its  applications in preclinical research and clinical development such as large molecules PK and TK studies  to be alternative methods to ligand binding assay (LBA) methods. The presentation will showcase the novel and combat-proven SMRM in the LC-MS/MS to audience。

Biography: 

Dr. Liang Tang is a pioneer in the field of electrospray ionization mass spectrometry (ESI-MS).  His early work with Professor Paul Kebarle on the research of electrospray ionization  mechanism set up the foundation of ESI-MS theory. The Kebarle-Tang Equation (Anal. Chem.  1991, 63, 2709-2715) depicted the relationship of major parameters in ESI-MS. Their broad  study from ions in solution to ions in the gas phase enriched the theory of analytical mass  spectrometry (Anal. Chem. 1993, 65, 972A-986A), and is frequently referred in literature to  explain many phenomena in LC-MS such as the matrix effect in bioanalysis. His recent interest  includes the application of analytical mass spectrometry in pharmaceutical research (e.g. J.  Chromatogr. B, 2022, 1193, 123165, and Anal. Bioanal. Chem. 2022, 414, 1933–1947). He is a  Senior Principal Scientist at SRI Biosciences.